Dr.Sci.(Biol.). Vavilov Institute of General Genetics, Laboratory of Stem Cell Biology
Genetics and epigenetics of stem cells
During last five years stem cells and cellular based technologies were thrice awarded by Nobel committee although they are not widely used in clinical practice yet now. There are a number of unresolved issues concerning stem cells genetic stability, differential potential and self-renewal. We are working with two types of human pluripotent stem cells: embryonic (ESC) and induced pluripotent stem cells (iPSC). We have established a number of ESC lines and used them to study epigenetic characteristics of pluripotency maintenance (Lagarkova et al., 2006) and genetic stability during long term cultivation (International Stem Cell Initiative et al., 2011). We have also developed robust protocols of human pluripotent stem cells differentiation into the cells of retina pigmented epithelium, endothelial cells, cardiomyocites etc (Lagarkova et al., 2008, Lagarkova et at., 2011). Utilizing reprogramming approaches we generated a number of iPSC lines and characterized their epigenetic profiles during reprogramming (Shutova et al., 2009, Lagarkova et al., 2010). iPSC generated from patients with genetically determined disorders such as Parkinson and Huntington diseases, amyotrophic lateral sclerosis, macula degeneration, myodistrophy and some others are currently under investigation to establish pathology mechanisms and to develop new treatments.
Main topics of the term
- Introduction in cell biology
- Introduction in embryology
- Stem cells
- Embryonic stem cells
- Gene knock-out technology
- Cell reprogramming
- Induced pluripotent stem cells
- Practical applications of reprogramming technologies
List of selected publications:
- Y.Sharovskaya, E.Philonenko, S.Kiselev, M.Lagarkova. De novo reestablishment of gap junctional intercellular communications during reprogramming to pluripotency and differentiation. Stem Cells Dev. 2012, Sep.20; 21(14):2623–9.
- International Stem Cell Initiative, K.Amps, P.Andrews, G.Anyfantis, S.Kiselev, M.Lagarkova. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage. Nat.Biotechnol. 2011, Nov.27; 29(12): 1132–44.
- С.Лагарькова, А.Г.Шилов, Н.И.Губанова, М.А.Прохорович, С.Л.Киселев. Гистогенез эмбриональных стволовых клеток человека in vitro в компоненты сетчатки глаза. Клеточные технологии в биологии и медицине. 2011, т.4, стр.203–206.
- E.Philonenko, M.Shutova, I.Chestkov, M.Lagarkova, S.Kiselev. Current progress and potential practical application for human pluripotent stem cells. Int.Rev.Cell Mol.Biol. 2011; 292: 153–96.
- A.Bogomazova, M.Lagarkova, L.Tskhovrebova, M.Shutova, S.Kiselev. Error-prone nonhomologous end joining repair operates in human pluripotent stem cells during late G2. Aging (Albany, NY). 2011, Jun.; 3(6): 584–96.
- M.Lagarkova, A.Eremeev, A.Svetlakov, N.Rubtsov, S.Kiselev. Human embryonic stem cell lines isolation, cultivation, and characterization. In Vitro Cell Dev.Biol.Anim. 2010, Apr.; 46(3–4): 284–93.
- M.Lagarkova, M.Shutova, A.Bogomazova, E.Vassina, E.Glazov, P.Zhang, A.Rizvanov, I.Chestkov, S.Kiselev. Induction of pluripotency in human endothelial cells resets epigenetic profile on genome scale. Cell Cycle. 2010, Mar.1; 9(5): 937–46.
- M.Lagarkova, P.Volchkov, E.Philonenko, K.Pfannkuche, M.Prokhorovich, T.Zabotina, J.Hescheler, S.Kiselev. CD 30 is a marker of undifferentiated human embryonic stem cells rather than a biomarker of transformed hESCs. Cell Cycle. 2008, Nov.15; 7(22): 3610–2.
- M.Shutova, A.Bogomazova, M.Lagarkova, S.Kiselev. Generation and characterization of human induced pluripotent stem cells. Acta Naturae. 2009, Jul; 1(2): 91–2.
- S.Korneev, E.Korneeva, M.Lagarkova, S.Kiselev, G.Critchley, M.O’Shea. Novel noncoding antisense RNA transcribed from human anti-NOS2A locus is differentially regulated during neuronal differentiation of embryonic stem cells. RNA. 2008, Oct; 14(10): 2030–7.
- M.Lagarkova, P.Volchkov, E.Philonenko, S.Kiselev. Efficient differentiation of hESCs into endothelial cells in vitro is secured by epigenetic changes. Cell Cycle. 2008.
- M.Lagarkova, P.Volchkov, A.Lyakisheva, E.Philonenko, S.Kiselev. Diverse epigenetic profile of novel human embryonic stem cell lines. Cell Cycle. 2006, Feb; 5(4): 416–20.